Preservation of salicaceae leaves for phytochemical analyses: Further assessment.
Identifieur interne : 004A37 ( Main/Exploration ); précédent : 004A36; suivant : 004A38Preservation of salicaceae leaves for phytochemical analyses: Further assessment.
Auteurs : R L Lindroth [États-Unis] ; P A KossSource :
- Journal of chemical ecology [ 0098-0331 ] ; 1996.
Abstract
The chemistry of the plant family Salicaceae has been of interest to researchers as diverse as chemical ecologists, chemosystematists, and paper chemists. Continuing the debate on proper methods for preservation of plant material prior to analysis, vacuum-drying was recently advocated, because freeze-drying may cause degradation of phenolic glycosides. This study was conducted to clarify the consequences of freeze-drying for foliar secondary chemicals and to evaluate the consequences of vacuum-drying for primary compounds (protein and carbohydrates). Leaves of quaking aspen (Populus tremuloides) were flash-frozen in liquid nitrogen and freeze-dried or vacuum-dried at room temperature. We then analyzed samples for levels of salicortin and tremulacin (phenolic glycosides), condensed tannins, nitrogen, soluble protein, sugars, and starch. Freeze-drying did not alter the concentrations of phenolic glycosides or tannins, relative to vacuum-drying. Freeze-drying did cause a small and inexplicable decline in nitrogen and soluble protein. Vacuum-drying, however, reduced starch concentrations by 38%. We suggest that the vacuum-drying method be used in studies in which carbohydrates are of no interest. For studies measuring carbohydrates, however, freeze-drying is a better alternative, and should effect no changes in levels of secondary compounds if samples are not allowed to thaw during the drying process.
DOI: 10.1007/BF02033584
PubMed: 24227583
Affiliations:
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Continuing the debate on proper methods for preservation of plant material prior to analysis, vacuum-drying was recently advocated, because freeze-drying may cause degradation of phenolic glycosides. This study was conducted to clarify the consequences of freeze-drying for foliar secondary chemicals and to evaluate the consequences of vacuum-drying for primary compounds (protein and carbohydrates). Leaves of quaking aspen (Populus tremuloides) were flash-frozen in liquid nitrogen and freeze-dried or vacuum-dried at room temperature. We then analyzed samples for levels of salicortin and tremulacin (phenolic glycosides), condensed tannins, nitrogen, soluble protein, sugars, and starch. Freeze-drying did not alter the concentrations of phenolic glycosides or tannins, relative to vacuum-drying. Freeze-drying did cause a small and inexplicable decline in nitrogen and soluble protein. Vacuum-drying, however, reduced starch concentrations by 38%. We suggest that the vacuum-drying method be used in studies in which carbohydrates are of no interest. For studies measuring carbohydrates, however, freeze-drying is a better alternative, and should effect no changes in levels of secondary compounds if samples are not allowed to thaw during the drying process. </div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">24227583</PMID><DateCompleted><Year>2013</Year><Month>11</Month><Day>15</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0098-0331</ISSN><JournalIssue CitedMedium="Print"><Volume>22</Volume><Issue>4</Issue><PubDate><Year>1996</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Journal of chemical ecology</Title><ISOAbbreviation>J Chem Ecol</ISOAbbreviation></Journal><ArticleTitle>Preservation of salicaceae leaves for phytochemical analyses: Further assessment.</ArticleTitle><Pagination><MedlinePgn>765-71</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/BF02033584</ELocationID><Abstract><AbstractText>The chemistry of the plant family Salicaceae has been of interest to researchers as diverse as chemical ecologists, chemosystematists, and paper chemists. Continuing the debate on proper methods for preservation of plant material prior to analysis, vacuum-drying was recently advocated, because freeze-drying may cause degradation of phenolic glycosides. This study was conducted to clarify the consequences of freeze-drying for foliar secondary chemicals and to evaluate the consequences of vacuum-drying for primary compounds (protein and carbohydrates). Leaves of quaking aspen (Populus tremuloides) were flash-frozen in liquid nitrogen and freeze-dried or vacuum-dried at room temperature. We then analyzed samples for levels of salicortin and tremulacin (phenolic glycosides), condensed tannins, nitrogen, soluble protein, sugars, and starch. Freeze-drying did not alter the concentrations of phenolic glycosides or tannins, relative to vacuum-drying. Freeze-drying did cause a small and inexplicable decline in nitrogen and soluble protein. Vacuum-drying, however, reduced starch concentrations by 38%. We suggest that the vacuum-drying method be used in studies in which carbohydrates are of no interest. For studies measuring carbohydrates, however, freeze-drying is a better alternative, and should effect no changes in levels of secondary compounds if samples are not allowed to thaw during the drying process. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lindroth</LastName><ForeName>R L</ForeName><Initials>RL</Initials><AffiliationInfo><Affiliation>Department of Entomology, University of Wisconsin, 53706, Madison, Wisconsin.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Koss</LastName><ForeName>P A</ForeName><Initials>PA</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Chem Ecol</MedlineTA><NlmUniqueID>7505563</NlmUniqueID><ISSNLinking>0098-0331</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>1995</Year><Month>06</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>1995</Year><Month>12</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>11</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>1996</Year><Month>4</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1996</Year><Month>4</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24227583</ArticleId><ArticleId IdType="doi">10.1007/BF02033584</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Chem Ecol. 1989 Jun;15(6):1899-917</Citation><ArticleIdList><ArticleId IdType="pubmed">24272191</ArticleId></ArticleIdList></Reference><Reference><Citation>Oecologia. 1993 Oct;95(4):495-498</Citation><ArticleIdList><ArticleId IdType="pubmed">28313289</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chem Ecol. 1989 Mar;15(3):979-92</Citation><ArticleIdList><ArticleId IdType="pubmed">24271900</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chem Ecol. 1984 Mar;10(3):499-520</Citation><ArticleIdList><ArticleId IdType="pubmed">24318555</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chem Ecol. 1989 Apr;15(4):1117-31</Citation><ArticleIdList><ArticleId IdType="pubmed">24271998</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chem Ecol. 1989 Sep;15(9):2335-46</Citation><ArticleIdList><ArticleId IdType="pubmed">24272421</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chem Ecol. 1995 Sep;21(9):1235-43</Citation><ArticleIdList><ArticleId IdType="pubmed">24234623</ArticleId></ArticleIdList></Reference><Reference><Citation>Oecologia. 1995 Jul;103(1):79-88</Citation><ArticleIdList><ArticleId IdType="pubmed">28306948</ArticleId></ArticleIdList></Reference><Reference><Citation>Planta Med. 1989 Feb;55(1):55-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17262254</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chem Ecol. 1990 Jun;16(6):1941-59</Citation><ArticleIdList><ArticleId IdType="pubmed">24263997</ArticleId></ArticleIdList></Reference><Reference><Citation>Oecologia. 1987 Nov;74(1):144-148</Citation><ArticleIdList><ArticleId IdType="pubmed">28310428</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 1980 Sep-Oct;28(5):947-52</Citation><ArticleIdList><ArticleId IdType="pubmed">7462522</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1985 Aug 16;229(4714):649-51</Citation><ArticleIdList><ArticleId IdType="pubmed">17739376</ArticleId></ArticleIdList></Reference><Reference><Citation>Oecologia. 1987 Oct;73(4):513-517</Citation><ArticleIdList><ArticleId IdType="pubmed">28311966</ArticleId></ArticleIdList></Reference><Reference><Citation>J Chromatogr. 1973 Sep 26;84(2):315-8</Citation><ArticleIdList><ArticleId IdType="pubmed">4745801</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Wisconsin</li></region></list><tree><noCountry><name sortKey="Koss, P A" sort="Koss, P A" uniqKey="Koss P" first="P A" last="Koss">P A Koss</name></noCountry><country name="États-Unis"><region name="Wisconsin"><name sortKey="Lindroth, R L" sort="Lindroth, R L" uniqKey="Lindroth R" first="R L" last="Lindroth">R L Lindroth</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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